Bioactive Component Analysis and in vitro Antioxidant Activities of Plantago Major L.

Abstract

Plantago major L. (P. major) is a plant used by people for medicinal purposes. In this study, ethanol extract was prepared after species identification of P. major plant growing in Varto district of Muş. Phenolic content and amount were determined by high pressure liquid chromatography (HPLC). Four different methods were used for antioxidant activities. As a result of the chemical content analysis of the plant, the highest amount of trans-p-cumaric acid (89.0 ng/μL), abscisic acid (11.4 ng/μL) and salicylic acid (8.20 ng/μL) were detected. When the antioxidant results were evaluated, it was observed that the extract scavenged DPPH and ABTS radicals with a power close to that of standard antioxidants. At the same time, the extract significantly reduced Fe+3 and Cu+2 ions. It was found that the activity of the samples generally increased with increasing absorbance. The fact that there are few studies on the antioxidant activity of P. major in the literature makes this study even more important. We hope that this study will shed light on future research on this species.