An investigation into the relationship between Taq1 and Apa1 polymorphisms of the vitamin D receptor gene and thedevelopment of osteoarthritis [osteoartrit gelişimi ve vitamin D reseptör geni Taqi ve Apai polimorfizmleri arasındaki i?lişki üzerine bir araştırma]

Abstract

Objectives: This study aims to determine whet her Vitamin D receptor (VDR) gene TaqI and ApaI polymorphisms are involved in the development of osteoarthritis in the Turkish population.Patients and methods: In this study, genomic DNA was isolated from 140 subjects (95 patients with osteoarthritis and 45 healthy controls). The DNA was amplified with specific primers by polymerase chain reaction and the restriction fragment length polymorphism (RFLP) technique was used to analyze the VDR gene TaqI and ApaI polymorphism genotypes. Polymerase chain reaction-RFLP products were assessed with and ultraviolet transilluminator by being exposed to agarose gel electrophoresis.Results: In terms of the genotype distributions and the allele frequencies of VDR gene TaqI and ApaI polymorphisms, there was no statistically significant difference (p>0.05) between the osteoarthritis patients and the controls. In addition, there was also no statistically significant difference between the genotypes and clinical characteristics of the patients or controls.Conclusion: In conclusion, the results of the present study suggest that VDR gene TaqI and ApaI polymorphisms are not involved in the development of osteoarthritis in the studied Turkish population. © 2011 Turkish League Against Rheumatism. All rights reserved.