Assessment of lactic acid bacteria isolated from traditional Mus Tulum cheese

Abstract

The dominant lactic acid bacteria (LAB) population in traditional Mud Tulum cheese, which is produced from unpasteurized sheep's milk, was identified during the ripening period (on the 1st, st , 45th, th , and 90th th day) using biochemical, phenotypic, and genotypic techniques. Additionally, the isolated LAB strains were assessed for their technological characteristics. Enterococcus spp. . accounted for 76.92 % of the sequenced isolates, while Lactiplantibacillus spp., . , Lactococcus spp., . , and Pediococcus spp. . represented 15.38 %, 6.15 %, and 1.54 % respectively. All Lactococcus strains as well as E. faecium FFH2 and Lb. plantarum FFH59 strains showed high acidification capacity. E. faecalis (FFH46 and FFH14), L. lactis subsp. lactis FFH51 and E. faecium FFH16 strains showed high proteolytic activity (2.0593-2.2452 FFA mmol/L). Two Enterococcus strains (E. E. faecium FFH12 and E. faecium FFH77) with potential bacteriocin production and antimicrobial activity were identified. The results indicated that Enterococcus spp. . was the dominant flora throughout the ripening period of Mud Tulum cheese, followed by Lactiplantibacillus spp.