Chemical constituent and radical scavenging antioxidant activity of Anthemis kotschyana Boiss

Abstract

Phenolic content and antioxidant activity of Anthemis kotschyana Boiss. var. discoidea (A. kotschyana) were reported in this study. The ethanol extract of Anthemis kotschyana (EEA) and the water extract of Anthemis kotschyana (WEA) were prepared and used for biochemical analyses. Radical scavenging antioxidant capacities of EEA and WEA were evaluated by DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging method. Another goal of the study was to evaluate the phenolic compositions of A. kotschyana by liquid chromatography and tandem mass spectrometry (LC-MS/MS). Rhamnetin (5.484 +/- 0.020 ppm; mu g/g extract) and quinic acid (2.251 +/- 0.012 ppm; mu g/g extract) were identified as major two compounds in the plant sample. This study will be a scientific base for further studies about A. kotschyana for plant biochemistry and plant-based pharmacological industry.