Preparation and Characterization of Silver-Loaded Magnetic Activated Carbon Produced from Crataegus Monogyna for Antimicrobial and Antioxidant Applications

Abstract

Secondary metabolites from several plant species have been used to cure various illnesses. Current advances allowed green synthesis nanoparticle manufacturing of metal salts from plant sources. This study involves binding activated carbon obtained from the Crataegus monogyna plant to magnetic nanoparticles and coating the resulting magnetic activated carbon nanocomposite with Ag ions (CMAC/MNPs-Ag) to produce a biomedical nanobiological material. Various techniques such as SEM, EDX, XRD, FTIR, UV-Vis, VSM, DLS, and zeta potential were used to characterize synthesized nanocomposites. CMAC/MNPs-Ag nanocomposite demonstrated activity in several processes of antioxidant activity tests. DPPH and CUPRAC activities of CMAC/MNPs-Ag nanocomposite were measured as 90.21 +/- 0.42 and 46.73 +/- 0.108 mg TE/g, respectively, while total phenolic content was measured as 27.15 +/- 0.381 mg GAE/g. Finally, the antibacterial activity of CMAC/MNPs-Ag nanocomposite was evaluated against Escherichia coli and Staphylococcus aureus by microdilution and disk diffusion techniques. The antimicrobial activity of CMAC/MNPs-Ag nanocomposite was determined using microdilution and disk diffusion techniques. For Escherichia coli, microdilution and disk diffusion were measured as 1.17 mu g mL-1 and 12 mm, respectively, while for Staphylococcus aureus, microdilution and disk diffusion were measured as 2.34 mu g mL-1 and 10 mm, respectively.